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:: Volume 13, Issue 2 (1-2025) ::
gebsj 2025, 13(2): 213-224 Back to browse issues page
Optimization of callus induction from ovary explants in desert Hyancinth (Cistanche tubulosa
Fatemeh Akbari , Fatemeh Zaker Tavallaie * , Masoumeh Salehi
Dept. of Plant Production and Genetics Engineering, Shirvan Agriculture Faculty, University of Bojnord, Bojnord, Iran. , f.zaker.t@um.ac.ir
Abstract:   (1040 Views)
Cistanche tubulosa, which is called Sonbol biyabani in Persian, is holoparasitic perennial plant, one of the most famous pasture and medicinal plants known as "desert ginseng". In this study, the callus formation of this plant was optimized. Initially, in order to determine the best disinfection treatment, plant parts were disinfected in five sodium hypochlorite treatments for 10 minutes and cultivated in MS culture medium. Also, the effect of activated charcoal and ascorbic acid on the control of browning of explants was investigated. Different explants were used for callus formation. To investigate the effect of plant growth regulators on plant callus formation, explants were cultivated on MS culture medium containing BAP at a concentration of 0.5 mg/L, kinetin at a concentration of 0.5 mg/L and 2.4.D in twenty-three levels with three replications. This experiment was conducted in a completely randomized design. After 10 days, the possibility of callus formation was examined for up to 2 months. The lowest level of contamination in the explants was observed in the treatment of 2% sodium hypochlorite and a disinfection time of 10 minutes. The treatment of 0.5 g/L of activated carbon plus 0.5 g/L of ascorbic acid was able to control browning well. The highest percentage of callus formation was obtained in the treatment of 0.5 mg/L of kinetin plus 0.5 mg/L of BAP and 8.5 mg/L of 2-4-D. The results of this study can be used in the future to perform indirect regeneration and also to produce active pharmaceutical ingredients in vitro using callus culture and cell suspension culture.
 
Keywords: Cistanche tubolosa, Callus induction, 2, 4-D, Browning
Full-Text [PDF 811 kb]   (200 Downloads)    
Type of Study: Research | Subject: Plant
Received: 2025/02/2 | Accepted: 2025/04/29 | Published: 2025/10/5
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Akbari F, Zaker Tavallaie F, Salehi M. Optimization of callus induction from ovary explants in desert Hyancinth (Cistanche tubulosa. gebsj 2025; 13 (2) :213-224
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Volume 13, Issue 2 (1-2025) Back to browse issues page
دوفصل نامه علمی-پژوهشی مهندسی ژنتیک و ایمنی زیستی Genetic Engineering and Biosafety Journal
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